Preparation, characterization and biological test of biodegradable composite tissue engineering scaffold

Abstract

We report fabrication of electro-spun polycaprolactone (PCL), gelatin and PCL–gelatin scaffolds in tissue engineering
based applications. The structural characterizations were investigated by fourier transform infrared spectroscopy (FT-IR)
and scanning electron microscopy (SEM) for the fabricated scaffolds. Pure PCL and gelatin scaffolds showed 3D
nano−fibrous porous structures at 20 wt. % and 30 wt. %, respectively. PCL−gelatin scaffold at 70:30 vol. % showed good
fibrous morphology without any structural discontinuities such as polymer beads, droplet formation and uneven fibers.
Cellular activities such as cell adhesion and growth on scaffold surfaces were performed with F11 human fibroblast cells
via MTT assay. Cell viability study showed that PCL−gelatin scaffold significantly improved the cell adhesion and
proliferation when compared to pure gelatin and PCL scaffolds. Thus, the PCL−gelatin scaffold reported in our work has
major prospective in the area of wound dressings, treatment of diabetic foot ulcers, implants and transdermal drug delivery