The Research of Abnormal Toxicity and Local Irritant Effect in the Draize Test of the Drug Furacilin, Concentrate for the Preparation of a Solution for Local and External Use

Abstract

Introduction: The research of abnormal toxicity and locally irritating effect of the drug Furacilin, concentrate for the
preparation of a solution for local and external use in comparison with the drug Furacilin, tablets for the preparation of the
solution, was carried out.
Objectives of the study: To determine the abnormal toxicity and locally irritating effect of the drug Furacilin in the Draize
test (OECD No. 405).
Methods: Abnormal toxicity was performed according to the requirements of the State Pharmacopoeia of the Russian
Federation (OFS.1.2.4.0004.15). The studied drugs were administered intraperitoneal volume of 0.5 ml (dose 25 mg/kg) at
a rate of 0.1 ml per second. The Draize test was performed by instillation into the conjunctival sac of rabbit medicals at a
concentration of 0.02% with a volume of 0.1 ml.
Results: After a single intraperitoneal administration of the studied drugs at a dose of 25 mg/kg, within 48 hours from the
moment of their introduction, the death of animals was not observed in any of the experimental groups.
After instillation of 0.02% solutions of 0.1 ml of the studied drugs to rabbits, and subsequent observation after 1, 24, 48 and
72 hours of changes in the conjunctiva, cornea and iris, chemosis was not registered. The number of points in all
parameters, in all experimental groups, did not exceed 0 points.
Conclusion: The obtained data indicate that Furacilin, a concentrate for the preparation of a solution for local and external
use 4 mg/ml in its parameters of Toxicological safety and the absence of local irritant action at the site of application,
comparable to the drug comparison Furacilin, tablets for the preparation of a solution for local and external use 20 mg.