The Cardio- and Endothelial Protective Effects of Ethyl Methyl Hydroxyl Pyridine Malate in Modeling L-Name Induced Nitric Oxide Deficiency

Abstract

Currently, endothelial dysfunction is considered as a predictor of a number of pathologies, including arterial hypertension,
ischemic heart disease, chronic heart failure, and is also a pathogenetic component of organ damage in diabetes, hypo
estrogenic and other conditions. The purpose of the study is an experimental study of the cardio and vasoprotective effects
of etoxidol under conditions of endothelial dysfunction.
Materials and methods: The study was performed on Wistar rats. The pharmacological properties of ethyl methyl
hydroxyl pyridine malate (etoxidol) were studied on the model of endothelial dysfunction caused by the blockade of NO
synthase on the background of the seven-day administration of L-NAME (25 mg / kg). To evaluate the effect of etoxidol on
endothelial function, a calculated index of functional vascular samples — the coefficient of endothelial dysfunction — was
used, and the morphological picture of the myocardium (determined the diameter of cardiomyocytes), carotid arteries
(intima / media ratio) and adrenal mass was studied.
Results: The seven-day administration of L-NAME led to the development of severe endothelial dysfunction. The use of
etoxidol in a dose of 50 mg / kg against a background of relatively high blood pressure values reduced QED by 30%, and
by 49% when using a dose of 90 mg / kg. With a relatively high diameter of cardiomyocytes in all studied groups receiving
L-NAME, a significant (p≤0.05) difference with the control was obtained with a dose of 90 mg / kg. When studying the
intima / media ratio in the carotid artery, a significant decrease in the ratio to the control was obtained in the group
receiving etoxidol at a dose of 90 mg / kg.
Conclusion: Etoxidol has a pronounced endothelial-, vaso- and cardio protective activity. The effectiveness of the drug is
dose-dependent: the best endothelium and cardio protective effects are achieved with a dose of 90 mg / kg.