Cardiac Fibroblasts Mitochondrial Activity in the Presence of Medicinal Preparations with Various Pharmacological Effects

Authors

  • Sergey V. Nadezhdin, Ekaterina V. Zubareva , Elizaveta. A. Brednikova , Evgeniya A. Movchan , Veronika S. Belyaeva , Vladimir Ya. Provotorov and Alexandr A. Stepchenko

Keywords:

Cardiac Fibroblasts, Mitochondria, MTT Assay, Antitumor Agent, Antibiotic, Antimetabolite, Antihypoxant, Cytotoxicity.

Abstract

The studies of cardiac fibroblasts mitochondria activity using fluorescence probe MitoTracker Red CMXRos (Thermo
Fisher Scientific, USA) and proliferation capacity with MTT assay (Thermo Fisher Scientific, USA) were carried out under
the influence of antihypoxant (trimetazidine) and anticancer (doxorubicin, cytosar) medicinal preparations. It was revealed
that medicinal preparations from the antitumor antibiotics group decreased mitochondria activity regardless of the cell state
unlike antitumor metabolites. Medicinal preparation trimetazidine increases cardiac fibroblasts mitochondrial activity.

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Published

19191919-August08-2323

Issue

Vol. 46 No. 4 (2019)

Section

Articles