Synthesis and effect of silver nanoparticle as a delivery systems and anticancer agent

Abstract

The field of nanotechnology meets the request for featuring new processes in the diagnosis and treatment of cancer.
Traditional processes for cancer treatments had included chemotherapy, surgery, and radiation. Early diagnosis and
treatment of cancer with these methods is still defying. The present study aims to innovative nanotechnology that can
overcome multidrug resistance (MDR), raise drug localization and efficiency. This done by applying a nanotechnology
technique to cancer cells which lead up to a new prospect for developing treatment methods on cancer cells. In this study
silver nanoparticles (Ag NPs) were synthesized by chemical approach in range of different temperatures (100°C- 120°C140°C- 160°C- 180°C- 200°C), the characteristics of Ag NPs where measured by many methods which include: atomic
force microscopy, UV-visible spectroscopy, zeta potential and raman spectroscopy techniques, the results of (Ag NP) tests
show spherical nanoparticle with size range from (8-18nm) and polarization from (-49.3mv)-(-52.48mv) which give an
indication of the stability of Ag NPs. The cytotoxicity of Ag NPs were tested in vitro by applying it on (L20B) cell lines
first as anticancer agent, second as drug delivery system by combining the nanoparticle with chemotherapy (Methotrexate),
third applied the chemotherapy (Methotrexate) on cell lines, the effect of Ag NPs and chemotherapy were measured by
MTT assay to evaluate their drug delivery activity and anticancer activity, results of MTT assay test shows high inhibition
rate of cell growth with Ag NPs as anticancer drug and Ag NP combined with chemotherapy as drug delivery system for
cancer therapy.