Effect of Low Level Laser on Changes in Physiological and Chemicals Values in the Rabbit Model (In Vivo)

Abstract

The irradiation effect using semiconductor laser, [635nm, and 5mW] which are low level laser, on the activity of cell ions
movement and blood gas analysis [BGA] in the animal model [rabbit] has been studied. Arterial blood gases[ABG] is an
important and useful test to monitor the basic parameters for blood gas concentration, gas control, and breathability control
and equilibrium conditions in animal model. Material & Methods: an Optical irradiation system has been designed and
utilized to increase the divergence of the laser beam emitted by a semiconductor laser; which is already of high divergence
by using divergent lens. The research animals used in the study have been divided into Four groups, including the control
group and another two groups, and one group immunized with CD19 antigen added to it immunosuppressant [Freund's
complete adjuvant's] hasn't exposed to the laser beam[non-irradiation], and the other group has been exposed to that laser
beam. The irradiation has been carried out through differed time periods for duration of 20 days. The animal general
activity has been monitored and level changed of blood gases [PH, Po2, Pco2, SaO2], electrolytes [Na+
, K+
, Ca+2,Ca+2[7.4],r,
Cl-
], Bicarbonates level HCO3
-
,Glocose and Lactate level as well as the level of other gaseous gases have been automated
analysis of blood chemistry & blood gases by EDAN i15. The Results: show a significant increase P≤0.05 in [Po2, SaO2]
and significant decreased P≤0.05 in Pco2at all exposure periods [1hr, 5hr, and 10hr], in addition show high significant in
pH at exposure [1hr, 5hr]. And appear significant changes P≤0.05 in [electrolytes, Lactate, HCT, and tHb] levels but no
significant change in glucose level. Conclusions: The effect of laser beam reaction 635nm laser shows the regulation of
ions movement, subsequently, there is no effect on cell voltage. These demonstrate regulation of cell membrane
permeability, PH regulation, and improvement of the oxygen transport ability of blood.